Composite

Part:BBa_K1747011:Design

Designed by: Harry Bennett   Group: iGEM15_LaVerne-Leos   (2015-08-26)


Fatty acid promoter - GFP - crt Z,E,B,I,Y - term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3347
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2883
    Illegal NgoMIV site found at 3013
    Illegal AgeI site found at 2098
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 734


Design Notes

There is no scar sequence in between the promoter and the first RBS


Source

All sequences were acquired from iGEM

References